پاورپوینت کنترل کیفی آزمایشهای سرولوژی (pptx) 65 اسلاید
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نوع فایل : PowerPoint (.pptx) ( قابل ویرایش و آماده پرینت )
تعداد اسلاید: 65 اسلاید
قسمتی از متن PowerPoint (.pptx) :
کنترول کیفی آزمایشهای سرولوژی
بنا م خداوند بخشنده مهربان
1. Labeled Immunoassay
Enzyme Linked Immuno Sorben Assay ( ELISA )
Radio Immuno Assay ( RIA )
Immuno Flurescent Antibody Technique ( IFAT )
Electrochemilominsance ( ECL )
2.Non Labeled Immunoassay.
Agglutination tests
Precipitation tests
Fluccolation tests
Agglutination Reactions
Direct agglutination
Passive agglutination
Agglutination inhibition
Ab + particulate Ag
Agglutination (clumping)
Direct agglutination
Example # 2 – Using bacteria (Ag) looking for Ab in serum.
Indirect or Passive agglutination
Results when inert particles are coated with soluble Ags which may react with Ab. Particles include latex, rbc’s, charcoal, etc.
Example – Ag attached to latex particle (known) + serum looking for (unknown) Ab. If Ab present, you get visible agglutination.
Reverse passive agglutination
Involves the adherence of Ab to inert particles which can then be used to detect the presence of Ag.
Example – latex particle coated with Ab (known) + serum looking for (unknown) Ag. If Ag present, then you get visible agglutination. (CRP test)
Agglutination inhibition - pos
Agglutination inhibition - neg