پاورپوینت استرپتوکک جلسه چهارم (pptx) 32 اسلاید
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بنام خدا
جلسه چهارم
استرپتوکک
انتروکک
GENERAL DISCUSSION
The streptococci are gram-positive cocci (0.5-1.0µm in diameter) which
occur in pairs and chains of varying length.
They are usually classified based on their hemolytic properties on blood agar and according to their serologic groups ( Lancefield grouping).
The streptococci are usually isolated on Sheep Blood agar ( BAP) ( Tryptic soy agar with ( 5-10 ) % Sheep blood ).
Hemolysis refers to is the lysis of the red blood cells in the agar surrounding
bacterial colonies and is a result of bacterial enzymes called hemolysins.
Reactions on blood agar are said to be beta, alpha, gamma, or double-zone:
Beta hemolysis refers to a clear, colorless zone surrounding the colony, where a complete lysis of the red blood cells by the hemolysins has occurred.
Alpha hemolysis appears as a zone of partial hemolysis surrounding
the colony, often accompanied by a greenish or brown discoloration due to oxidation of hemoglobin to met-hemoglobin by hydrogen peroxide
Gamma hemolysis refers to no hemolysis or discoloration of the agar
surrounding the colony.
Double-zone hemolysis refers to both a beta and an alpha zone of
hemolysis surrounding the colony.
Hemolysis on sheep blood agar: